Part:BBa_K2617010:Design

FRE_adhE: The fermentation regulatory element of Escherichia coli controls the expression

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

To get a good expression of this gene in E. coli, we did codon optimization before DNA synthesis.

Source

300bp upstream of the ackE gene, which is originally from Escherichia coli str. K-12 substr. MG1655